Clearance of protoporphyrin IX from mouse skin after topical application of 5-aminolevulinic acid and its methyl ester

Petras Juzenas; Roar Sorensen; Vladimir Iani; Johan Moan

doi:10.1117/12.339134

3 February 1999 Clearance of protoporphyrin IX from mouse skin after topical application of 5-aminolevulinic acid and its methyl ester

Petras Juzenas, Roar Sorensen, Vladimir Iani, Johan Moan

Author Affiliations +

Proceedings Volume 3563, Photochemotherapy of Cancer and Other Diseases; (1999) https://doi.org/10.1117/12.339134
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

The clearance of protoporphyrin IX (PpIX) from the skin of hairless BALB/c mice after topical application of 5- aminolevulinic acid (ALA) and its methyl ester (ALA-Me) was investigated. Creams containing 2 or 20% of ALA or ALA-Me were topically applied on spots of approximately 1 cm² for 12 hours. The PpIX fluorescence was detected by the means of a Perkin Elmer LS50B luminescence spectrometer equipped with a fiber-optic probe. The emission spectrum was identical with that of cell-bound PpIX. After 12 hours application of ALA and ALA-Me similar amounts of PpIX were found. After creme removal the ALA-induced PpIX fluorescence decayed with a half-life of about 20 hours (20% ALA cream). The ALA-Me-induced PpIX was faster cleared from the skin than ALA-induced PpIX, and had a half-life of about 7 hours (20% ALA-Me cream).

Citation Download Citation

Petras Juzenas, Roar Sorensen, Vladimir Iani, and Johan Moan "Clearance of protoporphyrin IX from mouse skin after topical application of 5-aminolevulinic acid and its methyl ester", Proc. SPIE 3563, Photochemotherapy of Cancer and Other Diseases, (3 February 1999); https://doi.org/10.1117/12.339134

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