Elastic-scattering spectroscopy for quantitative measurement of chemotherapy and PDT drug concentrations in vivo

Irving J. Bigio; Judith R. Mourant; Gerrit Los

doi:10.1117/12.336840

19 January 1999 Elastic-scattering spectroscopy for quantitative measurement of chemotherapy and PDT drug concentrations in vivo

Irving J. Bigio, Judith R. Mourant, Gerrit Los

Author Affiliations +

Proceedings Volume 3568, Optical Biopsies and Microscopic Techniques III; (1999) https://doi.org/10.1117/12.336840
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

We have applied elastic-scattering spectroscopy (ESS) for noninvasive, real-time in vivo measurement of the concentrations of certain drugs in tissue, utilizing a simple fiber-optic-probe spectroscopic system. The system uses a broadband light source, enabling the detection of compounds with absorption bands in most regions of the visible, as well as the NIR to 1700 nm. Subcutaneous tumors were grown in 4 Nude mice; the mice were treated with one of two chemotherapy agents, and the ESS system was used to perform pharmacokinetics measurements on the tumors following drug administration. Time histories of the drug concentrations in the tumors agreed with the known pharmacokinetics of the two drugs, and HPLC assays following sacrifice showed good correlation with the ESS values. Most photodynamic therapy agents and many chemotherapy drugs, including any that are not fluorescent, are ideal candidates for the ESS system. The measurement can be calibrated absolutely, and is not susceptible to problems associated with fluorescence assay methods.

Citation Download Citation

Irving J. Bigio, Judith R. Mourant, and Gerrit Los "Elastic-scattering spectroscopy for quantitative measurement of chemotherapy and PDT drug concentrations in vivo", Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); https://doi.org/10.1117/12.336840

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