FISH analysis of the arrangement of chromosomes in interphase nuclei using telomeric, centromeric, and DNA painting probes

Shamci Monajembashi; Eberhard Schmitt; Heike Dittmar; Karl-Otto Greulich

doi:10.1117/12.336825

19 January 1999 FISH analysis of the arrangement of chromosomes in interphase nuclei using telomeric, centromeric, and DNA painting probes

Shamci Monajembashi, Eberhard Schmitt, Heike Dittmar, Karl-Otto Greulich

Author Affiliations +

Proceedings Volume 3568, Optical Biopsies and Microscopic Techniques III; (1999) https://doi.org/10.1117/12.336825
Event: BiOS Europe '98, 1998, Stockholm, Sweden

Abstract

Fluorescence in situ hybridization is used to study the arrangement of chromosomes in interphase nuclei of unsynchronized human lymphocytes. DNA probes specific for telomeric DNA, centromeric (alpha) -satellite DNA and whole chromosomes 2, 7, 9 and X are employed. It is demonstrated that the shape of the chromosome territories is variable in cycling cells, for example, close to the metaphase chromosome homologues are arranged pairwise. Furthermore, the relative arrangement of chromosome homologues to each other is not spatially defined. Also, the relative orientation of centromeres and telomeres within a chromosome domain is variable.

Citation Download Citation

Shamci Monajembashi, Eberhard Schmitt, Heike Dittmar, and Karl-Otto Greulich "FISH analysis of the arrangement of chromosomes in interphase nuclei using telomeric, centromeric, and DNA painting probes", Proc. SPIE 3568, Optical Biopsies and Microscopic Techniques III, (19 January 1999); https://doi.org/10.1117/12.336825

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