Quantitative fluorescence spectroscopy of single molecules on surfaces

Martin Boehmer; Joerg Enderlein; Stefan Seeger

doi:10.1117/12.383339

21 April 2000 Quantitative fluorescence spectroscopy of single molecules on surfaces

Martin Boehmer, Joerg Enderlein, Stefan Seeger

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383339
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

In recent years, laser induced fluorescence detection and spectroscopy of single molecules (SMD) has seen a tremendous development. Besides fundamental research on individual molecular systems, practical applications of this sensitive detection technique for analytical and diagnostic purposes are becoming more and more important. For a wider applicability of the SMD technique it is desirable to detect not only the presence or absence of a molecule within a given detection volume,, but also t be able to quantify this fluorescence absolutely. Modified flow cytometry system for SMD that are ensuring such a quantifiable fluorescence detection of single molecules are already successfully applied for e.g. DNA fragment sizing. In our talk, we present a modified confocal detection set-up for performing SMD on surfaces that aims at a quantified detection of single molecule fluorescence. First experimental results presented and emerging problems are discussed.

Citation Download Citation

Martin Boehmer, Joerg Enderlein, and Stefan Seeger "Quantitative fluorescence spectroscopy of single molecules on surfaces", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383339

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