Synthesis, characterization, and application of fluorescent lipobeads for imaging and sensing in single cells

Kerry P. McNamara; Nitsa Rosenzweig; Zeev Rosenzweig

doi:10.1117/12.383342

21 April 2000 Synthesis, characterization, and application of fluorescent lipobeads for imaging and sensing in single cells

Kerry P. McNamara, Nitsa Rosenzweig, Zeev Rosenzweig

Author Affiliations +

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383342
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

This paper describes the synthesis and characterization of submicron phospholipid coated polystyrene particles, named lipobeads, with pH sensing capability. The phospholipids used to coat the particles are labeled with fluorescein and tetramethylrhodamine, which serves as a referencing fluorophore for increased accuracy of the pH measurements. The synthesis of the pH sensing lipobeads is realized by the covalent attachment of the fluorescent phospholipids to the surface of styrene-divinylbenzene micron or submicron sized particles. The pH dynamic range of the sensing particles is between pH 5.5 and 7 with a sensitivity of 0.1 pH units and they are photostable under the experimental conditions used in these studies. The fluorescent lipobeads are used to monitor pH changes in volume limited samples and to measure the pH of single macrophages. The lipobeads are ingested by the macrophages and directed to lysosomes, which are the cellular organelles involved in the phagocytosis process. Despite the high lyososomal levels of digestive enzymes and acidity, the absorbed particles remain stable for 6 hours in the cells when the cells are stored in a PBS buffer solution at pH 7.4.

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Kerry P. McNamara, Nitsa Rosenzweig, and Zeev Rosenzweig "Synthesis, characterization, and application of fluorescent lipobeads for imaging and sensing in single cells", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383342

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