Choline oxidase: a useful tool for high-throughput assays of acetylcholinesterase, phospholipase D, phosphatidylcholine-specific phospholipase C, and sphingomyelinase

Mingjie Zhou; Cailan Zhang; Richard P. Haugland

doi:10.1117/12.380507

22 March 2000 Choline oxidase: a useful tool for high-throughput assays of acetylcholinesterase, phospholipase D, phosphatidylcholine-specific phospholipase C, and sphingomyelinase

Mingjie Zhou, Cailan Zhang, Richard P. Haugland

Author Affiliations +

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380507
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Choline oxidase converts one molecule of choline to generate two molecules of H₂O₂. In a 1:1 reaction stoichiometry, horseradish peroxidase-catalyzed oxidation of the nonfluorescent and colorless H₂O₂ indicator, the Amplex Red reagent, by H₂O₂ produces the highly fluorescent resorufin, which has an excitation maximum at 563 nm and emission maximum at 587 nm. Using the Amplex Red reagent as an H₂O₂ indicator, assays of phospholipase D, phosphatidylcholine-specific phospholipase C, acetylcholine, acetylcholinesterase, and sphingomyelinase have been developed in choline oxidase- and HRP-coupled reactions. These assays have been developed in a microplate with a one-step procedure.

Citation Download Citation

Mingjie Zhou, Cailan Zhang, and Richard P. Haugland "Choline oxidase: a useful tool for high-throughput assays of acetylcholinesterase, phospholipase D, phosphatidylcholine-specific phospholipase C, and sphingomyelinase", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380507

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