Raman spectroscopic studies on bacteria

Kees Maquelin; Lin-P'ing Choo-Smith; Hubert Ph. Endtz; Hajo A. Bruining; Gerwin J. Puppels

doi:10.1117/12.409323

16 November 2000 Raman spectroscopic studies on bacteria

Kees Maquelin, Lin-P'ing Choo-Smith, Hubert Ph. Endtz, Hajo A. Bruining, Gerwin J. Puppels

Proceedings Volume 4161, Optical Biopsy and Tissue Optics; (2000) https://doi.org/10.1117/12.409323
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

Routine clinical microbiological identification of pathogenic micro-organisms is largely based on nutritional and biochemical tests. Laboratory results can be presented to a clinician after 2 - 3 days for most clinically relevant micro- organisms. Most of this time is required to obtain pure cultures and enough biomass for the tests to be performed. In the case of severely ill patients, this unavoidable time delay associated with such identification procedures can be fatal. A novel identification method based on confocal Raman microspectroscopy will be presented. With this method it is possible to obtain Raman spectra directly from microbial microcolonies on the solid culture medium, which have developed after only 6 hours of culturing for most commonly encountered organisms. Not only does this technique enable rapid (same day) identifications, but also preserves the sample allowing it to be double-checked with traditional tests. This, combined with the speed and minimal sample handling indicate that confocal Raman microspectroscopy has much potential as a powerful new tool in clinical diagnostic microbiology.

Citation Download Citation

Kees Maquelin, Lin-P'ing Choo-Smith, Hubert Ph. Endtz, Hajo A. Bruining, and Gerwin J. Puppels "Raman spectroscopic studies on bacteria", Proc. SPIE 4161, Optical Biopsy and Tissue Optics, (16 November 2000); https://doi.org/10.1117/12.409323

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available