Clinical-scale laser-based scanning and processing of live cells: selective photothermal killing of fluorescent tumor targets for autologous stem cell transplantation

Manfred R. Koller; Elie G. Hanania; Timothy Eisfeld; Robert A. O'Neal; Kevin M. Khovananth; Bernhard O. Palsson

doi:10.1117/12.424439

9 April 2001 Clinical-scale laser-based scanning and processing of live cells: selective photothermal killing of fluorescent tumor targets for autologous stem cell transplantation

Manfred R. Koller, Elie G. Hanania, Timothy Eisfeld, Robert A. O'Neal, Kevin M. Khovananth, Bernhard O. Palsson

Author Affiliations +

Proceedings Volume 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X; (2001) https://doi.org/10.1117/12.424439
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

High-dose chemotherapy, followed by autologous hematopoietic stem cell (HSC) transplantation, is widely used for the treatment of cancer. However, contaminating tumor cells within HSC harvests continue to be of major concern since re-infused tumor cells have proven to contribute to disease relapse. Many tumor purging methods have been evaluated, but all leave detectable tumor cells in the transplant and result in significant loss of HSCs. These shortcomings cause engraftment delays and compromise the therapeutic value of purging. A novel approach integrating automated scanning cytometry, image analysis, and selective laser-induced killing of labeled cells within a cell mixture is described here. Non-Hodgkin's lymphoma (NHL) cells were spiked into cell mixtures, and fluorochrome-conjugated antibodies were used to label tumor cells within the mixture. Cells were then allowed to settle on a surface, and as the surface was scanned with a fluorescence excitation source, a laser pulse was fired at every detected tumor cell using high-speed beam steering mirrors. Tumor cells were selectively killed with little effect on adjacent non-target cells, demonstrating the feasibility of this automated cell processing approach. This technology has many potential research and clinical applications, one example of which is tumor cell purging for autologous HSC transplantation.

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Manfred R. Koller, Elie G. Hanania, Timothy Eisfeld, Robert A. O'Neal, Kevin M. Khovananth, and Bernhard O. Palsson "Clinical-scale laser-based scanning and processing of live cells: selective photothermal killing of fluorescent tumor targets for autologous stem cell transplantation", Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); https://doi.org/10.1117/12.424439

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KEYWORDS

Tumors

Transplantation

Pulsed laser operation

Stem cells

Monoclonal antibodies

Laser processing

Luminescence

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