Confocal microscopic and spectroscopic investigation of autofluorescence of dictyosopharium pulchellum wood

Narahari V. Joshi; Honorio Medina; Maria Vielma

doi:10.1117/12.496590

2 November 2001 Confocal microscopic and spectroscopic investigation of autofluorescence of dictyosopharium pulchellum wood

Narahari V. Joshi, Honorio Medina, Maria Vielma

Author Affiliations +

Proceedings Volume 4431, Photon Migration, Optical Coherence Tomography, and Microscopy; (2001) https://doi.org/10.1117/12.496590
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

Dictyosphaerium puichellum Wood, a typical a fresh water alga, is known for photosynthesis and expected to have photoluminescence also. We have, therefore, carried out a confocal fluorescence microscopic and spectroscopic study of this species. 3-D image constructed from 50 optical sections clearly reveals that fluorescence is originated from only the outer layer and not from the central core. Spectral investigation shows that a strong and sharp peak is located at 684 nm. At this peak value, the power measurement was carried out and the number of photons emitted by alga in the solid angle 48.59 f was calculated and found to be 3.4*1010/ sec. The other significant feature of the responsible fluorophore is that the present photoluminescence has a very low quenching rate and hence the emitted radiation is observed for a very long time (5 12 sec.). This directly suggests that this fluorophore could have direct applications in dye related industries.

Citation Download Citation

Narahari V. Joshi, Honorio Medina, and Maria Vielma "Confocal microscopic and spectroscopic investigation of autofluorescence of dictyosopharium pulchellum wood", Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); https://doi.org/10.1117/12.496590

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available