Real-time atomic force fluorescence microscopy on living cells

Jens Struckmeier; Erk Klopp; Matthias Born; Martin R. Hofmann; Damaris Rink; David B. Jones; Stefan Ecke; Hans-Juergen Butt

doi:10.1117/12.446671

24 October 2001 Real-time atomic force fluorescence microscopy on living cells

Jens Struckmeier, Erk Klopp, Matthias Born, Martin R. Hofmann, Damaris Rink, David B. Jones, Stefan Ecke, Hans-Juergen Butt

Author Affiliations +

Proceedings Volume 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications; (2001) https://doi.org/10.1117/12.446671
Event: European Conferences on Biomedical Optics, 2001, Munich, Germany

Abstract

The reaction of bone cells after mechanical stimulation is a key issue in understanding the origins of osteoporosis. We investigate mechanical stimulation of osteoblasts and their reaction with a new combination of an atomic force apparatus with a fluorescence microscope. First measurements on the mechanically induced calcium response of osteoblasts are presented. The average threshold force for stimulation is 300nN and the dynamics of the calcium response is in the several tens of seconds range.

Citation Download Citation

Jens Struckmeier, Erk Klopp, Matthias Born, Martin R. Hofmann, Damaris Rink, David B. Jones, Stefan Ecke, and Hans-Juergen Butt "Real-time atomic force fluorescence microscopy on living cells", Proc. SPIE 4434, Hybrid and Novel Imaging and New Optical Instrumentation for Biomedical Applications, (24 October 2001); https://doi.org/10.1117/12.446671

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