Optical imaging fiber-based recombinant bacterial biosensor

Israel Biran; David M. Rissin; David R. Walt

doi:10.1117/12.456951

22 February 2002 Optical imaging fiber-based recombinant bacterial biosensor

Israel Biran, David M. Rissin, David R. Walt

Proceedings Volume 4576, Advanced Environmental Sensing Technology II; (2002) https://doi.org/10.1117/12.456951
Event: Environmental and Industrial Sensing, 2001, Boston, MA, United States

Abstract

A cell array biosensor was developed, composed of thousands of individual bacteria cells expressing a reporter gene that responds to the presence of environmental pollutants. The single cell array was produced by immobilizing the cells on an optical imaging fiber. A high-density array of microwells was fabricated on the optical imaging fiber's distal face by selectively etching the individual fibers cores. Each microwell was used to accommodate a single living bacterium, allowing simultaneous monitoring of the genetic responses of all the cells in the array. The optical imaging fiber cell array platform provides a powerful tool for the fabrication of whole cell biosensors for various environmental and industrial applications.

Citation Download Citation

Israel Biran, David M. Rissin, and David R. Walt "Optical imaging fiber-based recombinant bacterial biosensor", Proc. SPIE 4576, Advanced Environmental Sensing Technology II, (22 February 2002); https://doi.org/10.1117/12.456951

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