Multiple influence of Merocyanine-540 on spectra of perfused rat liver

Christine Mahlke; Marc Dammann; Maria-Theresa Stingl; Manfred D. Kessler

doi:10.1117/12.469467

5 June 2002 Multiple influence of Merocyanine-540 on spectra of perfused rat liver

Christine Mahlke, Marc Dammann, Maria-Theresa Stingl, Manfred D. Kessler

Proceedings Volume 4623, Functional Monitoring and Drug-Tissue Interaction; (2002) https://doi.org/10.1117/12.469467
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Complex multi-component systems are dominated in organs. Different functional structures could be partly represented as highly dynamic alterations of light scattering. In our institute we apply micro-lightguides and optical systems for monitoring subcellular structures. By creating 3D images after staining an isolated perfused rat liver with the potential sensitive fluorescence dye Merocyanine-540 we found an astonishing relation between morphological and functional aspects changeable by oxygenation or desoxygenation. Moreover, the simultaneous practice of 2D-spectroscopy could clarify cellular processes like e. g. potential changes. Merocyanine-540 spectra of stained rat liver have shown their maximum in light intensity at 596 +/- 2 nm in dependence on depolarisation, hyper- or repolarisation. In addition, we found lower peaks in difference spectra, which could be associated with the cytochromes aa₃, b and c. There has been an interesting correlation to the redox state of cytochromes during anoxic or oxic liver perfusion. Every peak has shown consistent oscillations with frequencies over 7/sec (420/min), which might be caused by membrane processes. In five experiments we compared functional and morphological aspects in 2D- and 3D-images of stained liver tissue.

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Christine Mahlke, Marc Dammann, Maria-Theresa Stingl, and Manfred D. Kessler "Multiple influence of Merocyanine-540 on spectra of perfused rat liver", Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); https://doi.org/10.1117/12.469467

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KEYWORDS

Liver

Tissues

Luminescence

Veins

Tissue optics

3D image processing

Absorption

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