Detection and imaging of the reconstituted pyropheophorbide-cholesterol oleate labeled low-density lipoprotein in the HepG2 tumor

Dana M. Blessington; Zhihong Zhang; Hui Li; Min Zhang; Lanlan Zhou; Jerry D. Glickson; Gang Zheng; Britton Chance

doi:10.1117/12.478190

29 July 2003 Detection and imaging of the reconstituted pyropheophorbide-cholesterol oleate labeled low-density lipoprotein in the HepG2 tumor

Dana M. Blessington, Zhihong Zhang, Hui Li, Min Zhang, Lanlan Zhou, Jerry D. Glickson, Gang Zheng, Britton Chance

Author Affiliations +

Proceedings Volume 4955, Optical Tomography and Spectroscopy of Tissue V; (2003) https://doi.org/10.1117/12.478190
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

We utilized the nude mouse model bearing the human hepatoblastoma G2 (HepG2) tumor and B-16 Murine Melanoma tumor to study the delivery and detection of the reconstituted Pyropheophorbide Cholesterol Oleate (r-pyroCE) molecular beacon. The delivery vehicle, low-density lipoprotein (LDL), labeled with the porphyrin derivative, was employed in response of the overexpression of LDL receptors in the HepG2 tumor. The B-16 melanoma tumor was also observed in this study for its overexpression of the LDL receptors. The tumors were imaged using the 3D low temperature scanner to produce images throughout several sliced sections of each tumor. The fluorescence signal of the pyropheophorbide was detected at 720nm when excited at 670nm in the tumor tissue. The uniform distribution of the signal in the HepG2 tumor shows extravasation of the beacon from the blood vessels. The B-16 tumor did not exhibit strong fluorescent signals and successful delivery as the HepG2 tumor outside the blood vessels and into the tumor tissue.

Citation Download Citation

Dana M. Blessington, Zhihong Zhang, Hui Li, Min Zhang, Lanlan Zhou, Jerry D. Glickson, Gang Zheng, and Britton Chance "Detection and imaging of the reconstituted pyropheophorbide-cholesterol oleate labeled low-density lipoprotein in the HepG2 tumor", Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); https://doi.org/10.1117/12.478190

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