Hybridization assay based on evanescent fluorescence excitation and collection

James J. Sumner; Robert U. Mmerole; Dimitra N. Stratis-Cullum; Hyunmin Yi; William E. Bentley; James B. Gillespie

doi:10.1117/12.486963

15 August 2003 Hybridization assay based on evanescent fluorescence excitation and collection

James J. Sumner, Robert U. Mmerole, Dimitra N. Stratis-Cullum, Hyunmin Yi, William E. Bentley, James B. Gillespie

Proceedings Volume 5085, Chemical and Biological Sensing IV; (2003) https://doi.org/10.1117/12.486963
Event: AeroSense 2003, 2003, Orlando, Florida, United States

Abstract

There is a great need for high throughput and sensitive sensors for genetic analysis. These sensors can be used for varied purposes from monitoring gene expression in organims to speciation of possible pathogens. Consequently, an instrument capable of these tasks would be a great benefit for food and water safety, medical diagnostics and defense of military and civilian populations from biological threats. This work examines the development of a hybridization-based biosensor using a novel tapered fiber optic rpobe. The immobilization of single-stranded, synthetic ologinucleotides utilizing aminoproplytriethoxysilane and glutaraldehyde was implemented on the fiber optic sensor. Hybridization takes place with a complementary analyte sequence followed by a fluorescent, labeled signaling probe to form a sandwich assay. Following hybridization, the fiber is interrogated with a diode laser source and the resulting fluorescence signal is detected using a miniature spectrometer.

Citation Download Citation

James J. Sumner, Robert U. Mmerole, Dimitra N. Stratis-Cullum, Hyunmin Yi, William E. Bentley, and James B. Gillespie "Hybridization assay based on evanescent fluorescence excitation and collection", Proc. SPIE 5085, Chemical and Biological Sensing IV, (15 August 2003); https://doi.org/10.1117/12.486963

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