Near-infrared Surface-Enhanced-Raman-Scattering (SERS) mediated discrimination of single, optically trapped, bacterial spores

Troy A. Alexander; Paul M. Pellegrino; James B. Gillespie

doi:10.1117/12.520996

15 March 2004 Near-infrared Surface-Enhanced-Raman-Scattering (SERS) mediated discrimination of single optically trapped bacterial spores

Troy A. Alexander, Paul M. Pellegrino, James B. Gillespie

Author Affiliations +

Proceedings Volume 5261, Smart Medical and Biomedical Sensor Technology; (2004) https://doi.org/10.1117/12.520996
Event: Optical Technologies for Industrial, Environmental, and Biological Sensing, 2003, Providence, RI, United States

Abstract

A novel methodology has been developed for the investigation of bacterial spores. Specifically, this method has been used to probe the spore coat composition of two different Bacillus stearothermophilus variants. This technique may be useful in many applications; most notably, development of novel detection schemes toward potentially harmful bacteria. This method would also be useful as an ancillary environmental monitoring system where sterility is of importance (i.e., food preparation areas as well as invasive and minimally invasive medical applications). This unique detection scheme is based on the near-infrared (NIR) Surface-Enhanced-Raman- Scattering (SERS) from single, optically trapped, bacterial spores. The SERS spectra of bacterial spores in aqueous media have been measured using SERS substrates based on ~60-nm diameter gold colloids bound to 3-Aminopropyltriethoxysilane derivatized glass. The light from a 787-nm laser diode was used to trap/manipulate as well as simultaneously excite the SERS of an individual bacterial spore. The collected SERS spectra were examined for uniqueness and the applicability of this technique for the strain discrimination of Bacillus stearothermophilus spores. Comparison of normal Raman and SERS spectra reveal not only an enhancement of the normal Raman spectral features but also the appearance of spectral features absent in the normal Raman spectrum.

Citation Download Citation

Troy A. Alexander, Paul M. Pellegrino, and James B. Gillespie "Near-infrared Surface-Enhanced-Raman-Scattering (SERS) mediated discrimination of single optically trapped bacterial spores", Proc. SPIE 5261, Smart Medical and Biomedical Sensor Technology, (15 March 2004); https://doi.org/10.1117/12.520996

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