Proteomic analyses of the acute tissue response for explant rabbit corneas and engineered corneal tissue models following in vitro exposure to 1540-nm laser light

Thomas E. Eurell; Thomas E. Johnson; Willliam P. Roach

doi:10.1117/12.529548

1 July 2004 Proteomic analyses of the acute tissue response for explant rabbit corneas and engineered corneal tissue models following in vitro exposure to 1540-nm laser light

Thomas E. Eurell, Thomas E. Johnson, Willliam P. Roach

Author Affiliations +

Proceedings Volume 5319, Laser Interaction with Tissue and Cells XV; (2004) https://doi.org/10.1117/12.529548
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Two-dimensional electrophoresis and histomorphometry were used to determine if equivalent protein changes occurred within native rabbit corneas and engineered corneal tissue models following in vitro exposure to single pulse, 1540 nm laser light operating at a pulse width of 0.8 milliseconds. Frozen sections of exposed tissues were processed to detect laser-induced protein changes. Isoelectric points, molecular weights and relative densities were used to characterize corneal proteins of interest that were then identified using MALDI-MS peptide fragment analysis. Increasing radiant exposures of corneal tissues were associated with progressively more severe necrosis of the epithelium and stroma in both the native and engineered tissues.

Citation Download Citation

Thomas E. Eurell, Thomas E. Johnson, and Willliam P. Roach "Proteomic analyses of the acute tissue response for explant rabbit corneas and engineered corneal tissue models following in vitro exposure to 1540-nm laser light", Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004); https://doi.org/10.1117/12.529548

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