Joint study of lipopolysaccharide suspensions with thermal lensing and optoacoustic methods

Nataliya V. Orlova; Anton V. Brusnichkin; Mikhail A. Proskurnin; Andrey V. Fokin; Oleg B. Ovchinnikov; Sergey V. Egerev

doi:10.1117/12.540677

12 July 2004 Joint study of lipopolysaccharide suspensions with thermal lensing and optoacoustic methods

Nataliya V. Orlova, Anton V. Brusnichkin, Mikhail A. Proskurnin, Andrey V. Fokin, Oleg B. Ovchinnikov, Sergey V. Egerev

Proceedings Volume 5320, Photons Plus Ultrasound: Imaging and Sensing; (2004) https://doi.org/10.1117/12.540677
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Pyrogens being introduced intravenously increase body temperature that leads to hazardous consequences and even to lethal outcome. One of the widespread pyrogen systems is presented by suspensions composed of bacterial endotoxins (or lypopolysaccharides, LPS). The aim of the work is to compare experimentally two methods for the determination of LPS at the submicrogram level and below. Both methods suppose that the LPS suspension is irradiated by a laser pulse. The thermal lens (TL) method (microsecond to millisecond irradiation cycle) detects LPS by a direct pick-up of the transient thermal field. The optoacoustic (OA) method (nanosecond laser pulses) has a potential to use non-thermal constitutents of the LPS response and to provide some selectivity of LPS detection with respect to optically uniform contaminants in the sample. In experiments, the selectivity was enhanced by means of analytical reagents, methylene blue and Stains All dyes. It was shown that both methods are mutually complementary. Then, their detectability potential increases and reaches 10 ppb if there occur ion pairs of LPS and cationic dye.

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Nataliya V. Orlova, Anton V. Brusnichkin, Mikhail A. Proskurnin, Andrey V. Fokin, Oleg B. Ovchinnikov, and Sergey V. Egerev "Joint study of lipopolysaccharide suspensions with thermal lensing and optoacoustic methods", Proc. SPIE 5320, Photons Plus Ultrasound: Imaging and Sensing, (12 July 2004); https://doi.org/10.1117/12.540677

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