Comparative analysis of infrared fluorescence generation in multiphoton spectroscopy

Philippe Legros; Daniel Choquet; Eric P. Mottay; Nelly Deguil; Francois Salin

doi:10.1117/12.533966

21 June 2004 Comparative analysis of infrared fluorescence generation in multiphoton spectroscopy

Philippe Legros, Daniel Choquet, Eric P. Mottay, Nelly Deguil, Francois Salin

Proceedings Volume 5323, Multiphoton Microscopy in the Biomedical Sciences IV; (2004) https://doi.org/10.1117/12.533966
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

We have applied a new, 1030 nm wavelength, infrared diode-pumped femtosecond laser source to multiphoton microscopy, and present comparative results on the efficiency of fluorescence generation versus wavelength for several fluorophores. It is shown that an emission wavelength of 1030 nm is optimal both for GFP and DsRed excitation.

Citation Download Citation

Philippe Legros, Daniel Choquet, Eric P. Mottay, Nelly Deguil, and Francois Salin "Comparative analysis of infrared fluorescence generation in multiphoton spectroscopy", Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); https://doi.org/10.1117/12.533966

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