Quantification of integrin dynamics on cell membranes with fluorescence correlation spectroscopy

Bryon E. Wright; Vladimir Hlady

doi:10.1117/12.528046

21 June 2004 Quantification of integrin dynamics on cell membranes with fluorescence correlation spectroscopy

Bryon E. Wright, Vladimir Hlady

Proceedings Volume 5323, Multiphoton Microscopy in the Biomedical Sciences IV; (2004) https://doi.org/10.1117/12.528046
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Integrins are an important and highly conserved class of extracellular matrix binding membrane bound receptors that provide a functional linkage between cells and their environment. The excellent spatial resolution and short observation time of a modern fluorescence correlation spectroscopy (FCS) instrument is uniquely suited to the study of quantitative differences in integrin behavior on different parts of a single cell. We hypothesize that the application of FCS to the study of these integrin dynamics on the membranes of nerve cell growth cones will give previously unavailable quantitative insight into nerve cell guidance. Accordingly, we have characterized the application of two integrin-binding small peptide-based FCS analytes to primary rat dorsal root ganglion cells. It results that neither the RGD-based nor the SIKVAV-based analyte exhibited specific association with primary rat dorsal root ganglion growth cone membranes in a range that is accessible to FCS observation. However, the techniques and instrumentation developed for these experiments will be useful for evaluating additional integrin ligands.

Citation Download Citation

Bryon E. Wright and Vladimir Hlady "Quantification of integrin dynamics on cell membranes with fluorescence correlation spectroscopy", Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); https://doi.org/10.1117/12.528046

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