Nanostructured thin films for fluorescence enhancement

Sandrine Leveque-Fort; Emmanuel Fort; Eric Le Moal; Jean-Pierre Lacharme; Marie-Pierre Fontaine-Aupart; Christian Ricolleau

doi:10.1117/12.529909

16 June 2004 Nanostructured thin films for fluorescence enhancement

Sandrine Leveque-Fort, Emmanuel Fort, Eric Le Moal, Jean-Pierre Lacharme, Marie-Pierre Fontaine-Aupart, Christian Ricolleau

Author Affiliations +

Proceedings Volume 5327, Plasmonics in Biology and Medicine; (2004) https://doi.org/10.1117/12.529909
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

Fluorescence is widely used as a spectroscopic tool or for biomedical imaging, in particular for DNA chips. Nanostructured metallic substrates permit to locally enhance the fluorescence signal which offer the possibility both to detect very small fluorophore concentrations and to trace precisely the bio-markers. We have developed substrates made of silver or gold nanoparticles covered with a spacer layer of alumina. Double metallic and dielectric gradients permit to directly map the fluorescence enhancement factor and to determine the best condition for maximum enhancement. One and two photons excitations are studied. Fluorescence enhancement reaches two orders of magnitudes. Lifetime measurements reveal additional information on the decay channels induced by the nanoparticle presence.

Citation Download Citation

Sandrine Leveque-Fort, Emmanuel Fort, Eric Le Moal, Jean-Pierre Lacharme, Marie-Pierre Fontaine-Aupart, and Christian Ricolleau "Nanostructured thin films for fluorescence enhancement", Proc. SPIE 5327, Plasmonics in Biology and Medicine, (16 June 2004); https://doi.org/10.1117/12.529909

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available