Ns-scale time-resolved laser induced fluorescence imaging for detection of fecal contamination on apples

Moon S. Kim; Alan M. Lefcourt; Yud-Ren Chen

doi:10.1117/12.582042

19 November 2004 Ns-scale time-resolved laser induced fluorescence imaging for detection of fecal contamination on apples

Moon S. Kim, Alan M. Lefcourt, Yud-Ren Chen

Author Affiliations +

Proceedings Volume 5587, Nondestructive Sensing for Food Safety, Quality, and Natural Resources; (2004) https://doi.org/10.1117/12.582042
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

Our laboratory has been utilizing fluorescence techniques as a potential means for detection of quality and wholesomeness of food products. A system with a short pulse light source such as a laser coupled with a gated detector can be used to harvest fluorescence in ambient light conditions from biological samples with relatively low fluorescence yields. We present a versatile multispectral laser-induced fluorescence (LIF) imaging system capable of ns-scale time resolved fluorescence. The system is equipped with a tunable pulse laser system that emits in the visible range from 410 nm to 690 nm. Ns-scale, time-dependent multispectral fluorescence emissions of apples contaminated with a range of diluted cow feces were acquired. Four spectral bands, F670, F680, F685 and F730, centered near the emission peak wavelengths of the major constituents responsible for the red fluorescence emissions from apples artificially contaminated with cow feces were examined to determine a suitable single red fluorescence band and optimal ns-gate window for detection of fecal contamination on apples. The results based on the ns decay curves showed that 670 nm with 10 nm full width at half maximum (FWHM) at a gate-delay of 4 ns from the laser excitation peak provided the greatest differences in time-dependent fluorescence responses between feces contaminated spots and apples surfaces.

Citation Download Citation

Moon S. Kim, Alan M. Lefcourt, and Yud-Ren Chen "Ns-scale time-resolved laser induced fluorescence imaging for detection of fecal contamination on apples", Proc. SPIE 5587, Nondestructive Sensing for Food Safety, Quality, and Natural Resources, (19 November 2004); https://doi.org/10.1117/12.582042

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