In situ synthesis of oligonucleotides on plasma-modified PTFE for fabricating DNA chips

Nongyue He; Jianxin Tang; Libo Nie; Hong Chen

doi:10.1117/12.573464

29 December 2004 In situ synthesis of oligonucleotides on plasma-modified PTFE for fabricating DNA chips

Nongyue He, Jianxin Tang, Libo Nie, Hong Chen

Proceedings Volume 5593, Nanosensing: Materials and Devices; (2004) https://doi.org/10.1117/12.573464
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

Oligonucleotide probe arrays were in-situ synthesized on the H₂/N₂ plasma modified poly(terafluoroethylene) (PTFE) surface via micro-fluidic channels connected with an automated synthesizer. A contact angle measurement of water droplets was used to ascertain the hydrophilicity of the surface. X-ray photoelectron spectroscopy (XPS) analysis confirmed the presence of polar amino groups on the surface. Ultra-violet (UV) spectrum analysis indicated that the surface showed a coupling efficiency higher than 98% for in situ synthesis of oligonucleotides arrays. The probe array specificity was discriminated by hybridizing with fluorescent target sequencyes. Oligonucleotide probe arrays on modified PTFE surface showed high stability and durability after repetitive denaturing and hybridizing. The results implied the plasma modified PTFE surface was extremely stable, performed well in DNA hybridization assays and could service as a good substrate for high-density oligonucleotide array synthesis.

Citation Download Citation

Nongyue He, Jianxin Tang, Libo Nie, and Hong Chen "In situ synthesis of oligonucleotides on plasma-modified PTFE for fabricating DNA chips", Proc. SPIE 5593, Nanosensing: Materials and Devices, (29 December 2004); https://doi.org/10.1117/12.573464

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