The effect of low-intensity laser therapy on wound healing in Streptozotocin-induced diabetic rats

Sylvia Bicahlo Rabelo; Antonio Guillermo Jose Balbin Villaverde; Miguel A. Castilho Salgado; Milene da Silva Melo; Renata Amadei Nicolau; Marcos Tadeu Tavares Pacheco

doi:10.1117/12.589345

21 October 2004 The effect of low-intensity laser therapy on wound healing in Streptozotocin-induced diabetic rats

Sylvia Bicahlo Rabelo, Antonio Guillermo Jose Balbin Villaverde, Miguel A. Castilho Salgado, Milene da Silva Melo, Renata Amadei Nicolau, Marcos Tadeu Tavares Pacheco

Author Affiliations +

Proceedings Volume 5622, 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications; (2004) https://doi.org/10.1117/12.589345
Event: 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications, 2004, Porlamar, Venezuela

Abstract

Diabetes Mellitus is a condition that results in a delay of the wound healing process, that is associated with an insufficient production of collagen, a decrease of the amount of collagen fibrils and deficient blood flow in the wound area. It is sugested that Low Intensity Laser Therapy acts by improving wound healing in normal organisms, accelerating tissue regeneration. The aim of this work was to investigate the biostimulatory effect of the HeNe laser irradiation, at 632.8 nm, on wound healing in 15 male rats suffering from diabetes induced by Streptozotocin, compared to 15 control diabetic animals. Irradiation parameters were: laser power of 15mW, exposition time of 17 s., irradiated area of 0.025 cm² and laser energy density of 10 J/cm². Full-thickness skin squared samples, with 5 mm of non-injured tissue around the wound, were obtained at 4, 7 and 15 days after wounding procedure (5 treated and 5 control animals each time). The histopathologic analysis performed by haematoxylin-cosin staining. Results suggested that the irradiation of diabetic rats was efficient for wound healing. Treated group presented better quality of the wound tissues by the macroscopic observation than control group and the microscopic analysis demonstrated that treated animals had better histopathologic evaluation than non treated.

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Sylvia Bicahlo Rabelo, Antonio Guillermo Jose Balbin Villaverde, Miguel A. Castilho Salgado, Milene da Silva Melo, Renata Amadei Nicolau, and Marcos Tadeu Tavares Pacheco "The effect of low-intensity laser therapy on wound healing in Streptozotocin-induced diabetic rats", Proc. SPIE 5622, 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications, (21 October 2004); https://doi.org/10.1117/12.589345

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