BioMEMS for high-throughput handling and microinjection of embryos

Ralph W. Bernstein; Matthew Scott; Olav Solgaard

doi:10.1117/12.584626

30 December 2004 BioMEMS for high-throughput handling and microinjection of embryos

Ralph W. Bernstein, Matthew Scott, Olav Solgaard

Proceedings Volume 5641, MEMS/MOEMS Technologies and Applications II; (2004) https://doi.org/10.1117/12.584626
Event: Photonics Asia, 2004, Beijing, China

Abstract

Technologies for handling, sorting, and positioning of embryos are increasingly important in biomedicine. In this paper the status for ongoing projects aimed at developing instrumentation for high-throughput treatment of embryos is reviewed. Techniques for positioning of Drosophila (fruit-fly) embryos in 2-D arrays for use in microinjection experiments are especially focused. A method based on fluidic micro assembly is discussed, and important parameters such as immobilization yield, the number of misplaced embryos, and adhesion force of the embryos are reported. A model for the assembly process is described, and simulation results are in good agreement with adhesion force measurements. A fully automated MEMS based system for fruit-fly embryo injection has recently been demonstrated at Stanford University. The first experiments with double-stranded RNA injection proved successful, and the expected genetic modification of the embryos was observed.

Citation Download Citation

Ralph W. Bernstein, Matthew Scott, and Olav Solgaard "BioMEMS for high-throughput handling and microinjection of embryos", Proc. SPIE 5641, MEMS/MOEMS Technologies and Applications II, (30 December 2004); https://doi.org/10.1117/12.584626

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