Characterization of thermally induced transitions of collagen using second harmonic generation (SHG) microscopy

Yen Sun; Chih-Yuan Hsiao; Shu-Wen Teng; Wen Lo; Hsian-Yuan Tan; Sung-Jan Lin; Gwo-Jen Jan; Wei-Chou Lin; Shiou-Hwa Jee; Chen-Yuan Dong

doi:10.1117/12.587176

14 April 2005 Characterization of thermally induced transitions of collagen using second harmonic generation (SHG) microscopy

Yen Sun, Chih-Yuan Hsiao, Shu-Wen Teng, Wen Lo, Hsian-Yuan Tan, Sung-Jan Lin, Gwo-Jen Jan, Wei-Chou Lin, Shiou-Hwa Jee, Chen-Yuan Dong

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Proceedings Volume 5698, Thermal Treatment of Tissue: Energy Delivery and Assessment III; (2005) https://doi.org/10.1117/12.587176
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

As a major component of the connective tissues, collagen fibers are responsible for various physiological functions inside the body. They provide support for the skin, partial focusing through the cornea, and coordinate movements via tendons, ligaments, and cartilages. In many medical procedures, thermal reorganization of the collagen structure is inevitable or desired. Therefore, the optimization of the therapeutic values of these procedures requires the characterization of thermal changes to collagen fibers. In this presentation, we use multiphoton microscopy to achieve this task. We will show that second harmonic generation (SHG) microscopy can characterize the thermally altered states of collagen and that they have potentials to be used in imaging applications in vivo.

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Yen Sun, Chih-Yuan Hsiao, Shu-Wen Teng, Wen Lo, Hsian-Yuan Tan, Sung-Jan Lin, Gwo-Jen Jan, Wei-Chou Lin, Shiou-Hwa Jee, and Chen-Yuan Dong "Characterization of thermally induced transitions of collagen using second harmonic generation (SHG) microscopy", Proc. SPIE 5698, Thermal Treatment of Tissue: Energy Delivery and Assessment III, (14 April 2005); https://doi.org/10.1117/12.587176

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KEYWORDS

Collagen

Second-harmonic generation

Harmonic generation

Microscopy

Multiphoton microscopy

Optical fibers

Tissues

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