Growth of crystals in optical tweezers

Ursula Gibson; Wolfgang Singer; Timo Nieminen; Norman Heckenberg; Halina Rubinsztein-Dunlop

doi:10.1117/12.616739

26 August 2005 Growth of crystals in optical tweezers

Ursula Gibson, Wolfgang Singer, Timo Nieminen, Norman Heckenberg, Halina Rubinsztein-Dunlop

Proceedings Volume 5930, Optical Trapping and Optical Micromanipulation II; 593014 (2005) https://doi.org/10.1117/12.616739
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

We report here on the use of optical tweezers in the growth and manipulation of protein and inorganic crystals. Sodium chloride and hen egg-white lysozyme crystals were grown in a batch process, and then seeds from the solution were introduced into the optical tweezers. The regular and controllable shape and the known optical birefringence in these structures allowed a detailed study of the orientation effects in the beam due to both polarization and gradient forces. Additionally, we determined that the laser tweezers could be used to suspend a crystal for three-dimensional growth under varying conditions. Studies included increasing the protein concentration, thermal cycling, and a diffusion-induced increase in precipitant concentration. Preliminary studies on the use of the tweezers to create a localized seed for growth from polyethylene oxide solutions are also reported.

Citation Download Citation

Ursula Gibson, Wolfgang Singer, Timo Nieminen, Norman Heckenberg, and Halina Rubinsztein-Dunlop "Growth of crystals in optical tweezers", Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 593014 (26 August 2005); https://doi.org/10.1117/12.616739

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