Development of an electrochemical biosensor without a sandwich assay

James J. Sumner; Kevin W. Plaxco; Carl D. Meinhart; Hyongsok Soh

doi:10.1117/12.630748

11 November 2005 Development of an electrochemical biosensor without a sandwich assay

James J. Sumner, Kevin W. Plaxco, Carl D. Meinhart, Hyongsok Soh

Proceedings Volume 6007, Smart Medical and Biomedical Sensor Technology III; 600706 (2005) https://doi.org/10.1117/12.630748
Event: Optics East 2005, 2005, Boston, MA, United States

Abstract

The combination of electrochemistry with microfluidic sample processing is a viable option to reduce the size, logistics load and power consumption of biosensors. Modern microfluidics technology makes it possible to perform sample clean-up, PCR, sample concentration and transduction on the same disposable chip. This presentation will discuss two novel electrochemical techniques which do not require a sandwich assay and can be employed on a disposable microfluidic chip, reducing logistics load and microfluidic complexity. Transduction is achieved via an electrochemical DNA hybridization sensor similar to a molecular beacon removing the need for a sandwich assay also referred to as E-DNA. The sensor is designed where a DNA stem-loop structure is immobilized on a gold electrode with a redox label held close to the surface. Upon hybridization the stem-loop opens and the label pulls away from the surface so that current cannot flow to the electrode under positive bias. This paper will primarily discuss experiments trying to understand the hybridization event and effect of surface morphology on electrochemical signal transduction.

Citation Download Citation

James J. Sumner, Kevin W. Plaxco, Carl D. Meinhart, and Hyongsok Soh "Development of an electrochemical biosensor without a sandwich assay", Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 600706 (11 November 2005); https://doi.org/10.1117/12.630748

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available