In vivo time-resolved autofluorescence measurements on human skin

Kamal M. Katika; Laurent Pilon; Katrina Dipple M.D.; Seymour Levin M.D.; Jennifer Blackwell; Halil Berberoglu

doi:10.1117/12.647149

22 February 2006 In vivo time-resolved autofluorescence measurements on human skin

Kamal M. Katika, Laurent Pilon, Katrina Dipple M.D., Seymour Levin M.D., Jennifer Blackwell, Halil Berberoglu

Proceedings Volume 6078, Photonic Therapeutics and Diagnostics II; 60780L (2006) https://doi.org/10.1117/12.647149
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

In this paper we present preliminary results obtained from fluorescence lifetime measurements on human skin using time-correlated single photon counting (TCSPC) techniques. Human skin was exposed to light from a pulsed LED of 700 ps pulse width at a wavelength of 375 nm and fluorescence decays were recorded at four different emission wavelengths (442, 460, 478 and 496 nm) using a photomultiplier tube (PMT) coupled to a monochromator. Measurements were carried out on the left and right palms of subjects recruited for the study after obtaining consent using a UCLA IRB approved consent form. The subjects recruited consisted of 18 males and 17 females with different skin complexions and ages ranging from 10 to 70 years. In addition, a set of experiments were also performed on various locations including the palm, the arm and the cheek of a Caucasian subject. The fluorescence decays thus obtained were fit to a three-exponential decay model in all cases and were approximately 0.4, 2.7 and 9.4 ns, respectively. The variations in these lifetimes with location, gender, skin complexion and age are studied. It is speculated that the shorter lifetimes correspond to free and bound NADH while the longer lifetime is due to AGE crosslinks.

Citation Download Citation

Kamal M. Katika, Laurent Pilon, Katrina Dipple M.D., Seymour Levin M.D., Jennifer Blackwell, and Halil Berberoglu "In vivo time-resolved autofluorescence measurements on human skin", Proc. SPIE 6078, Photonic Therapeutics and Diagnostics II, 60780L (22 February 2006); https://doi.org/10.1117/12.647149

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