Using FLIM in the study of permeability barrier function of aged and young skin

P. Xu; E. H. Choi; M. Q. Man; D. Crumrine; T. Mauro; P. Elias

doi:10.1117/12.660985

23 February 2006 Using FLIM in the study of permeability barrier function of aged and young skin

P. Xu, E. H. Choi, M. Q. Man, D. Crumrine, T. Mauro, P. Elias

Proceedings Volume 6089, Multiphoton Microscopy in the Biomedical Sciences VI; 60890Q (2006) https://doi.org/10.1117/12.660985
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Aged skin commonly is afflicted by inflammatory skin diseases or xerosis/eczema that can be triggered or exacerbated by impaired epidermal permeability barrier homeostasis. It has been previously described a permeability barrier defect in humans of advanced age (> 75 years), which in a murine analog >18 mos, could be attributed to reduced lipid synthesis synthesis. However, the functional abnormality in moderately aged mice is due not to decreased lipid synthesis, but rather to a specific defect in stratum corneum (SC) acidification causing impaired lipid processing processing. Endogenous Na⁺/H⁺ antiporter (NHE1) level was found declined in moderately aged mouse epidermis. This acidification defect leads to perturbed permeability barrier homeostasis through more than one pathways, we addressed suboptimal activation of the essential, lipid-processing enzyme, β-glucocerebrosidase (BGC) is linked to elevated SC pH. Finally, the importance of the epidermis acidity is shown by the normalization of barrier function after exogenous acidification of moderately aged skin.

Citation Download Citation

P. Xu, E. H. Choi, M. Q. Man, D. Crumrine, T. Mauro, and P. Elias "Using FLIM in the study of permeability barrier function of aged and young skin", Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 60890Q (23 February 2006); https://doi.org/10.1117/12.660985

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available