Plant antigenotoxicity assay using red fluorescent protein (RFP)-expressing bacteria

Katheryn Mandap; Kevim David; Fortunato Sevilla III; Amelita Bartolome

doi:10.1117/12.643928

23 February 2006 Plant antigenotoxicity assay using red fluorescent protein (RFP)-expressing bacteria

Katheryn Mandap, Kevim David, Fortunato Sevilla III, Amelita Bartolome

Proceedings Volume 6098, Genetically Engineered Probes for Biomedical Applications; 60980I (2006) https://doi.org/10.1117/12.643928
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Analytical biophotonics techniques such as steady-state fluorescence spectroscopy and fluorescence microscopy have been proven to be important tools in the study of genetically engineered bacterial sensors for plant antigenotoxicity. The assay involves the use of E. coli RS4U tagged with the red fluorescent protein (RFP). The cells emit red fluoresce in direct proportion to the genotoxicant present. Antigenotoxicity is seen as an act of preventing the DNA-damage induced expression of RFP. Thus, co-treatment of the cells with the genotoxicant and antigenotoxicant plant samples resulted to the reduction of the RFP fluorescence.

Citation Download Citation

Katheryn Mandap, Kevim David, Fortunato Sevilla III, and Amelita Bartolome "Plant antigenotoxicity assay using red fluorescent protein (RFP)-expressing bacteria", Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 60980I (23 February 2006); https://doi.org/10.1117/12.643928

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