Multipopulation desaggregation behavior of zFP538 upon dilution

Vadim A. Korolenko; Evgeny Evtushenko; Alexander L. Rusanov; Nadya Zubova; Ilya N. Kurochkin; Alexander P. Savitsky

doi:10.1117/12.650601

23 February 2006 Multipopulation disaggregation behavior of zFP538 upon dilution

Vadim A. Korolenko, Evgeny Evtushenko, Alexander L. Rusanov, Nadya Zubova, Ilya N. Kurochkin, Alexander P. Savitsky

Proceedings Volume 6098, Genetically Engineered Probes for Biomedical Applications; 60980N (2006) https://doi.org/10.1117/12.650601
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Practically all fluorescent proteins from corals are oligomerized or aggregated in solutions. For zFP538 it was demonstrated that intensity of fluorescence and pH-profile change upon dilution that was explained by the dissociation of aggregates. Particle size distribution of zFP538 aggregates adsorbed on the surface of graphite from solutions in the concentration range 10^-7 - 10^-9 M was studied by AFM. Multi-population size distribution was observed for all three protein concentrations. Within one concentration, size distribution is characterized by three populations: small, medium, and large. For 10^-7M concentration, the largest size is 10 times less than that in the 10^-5M solution or solid film. For 10^-8M concentration, the maximum population is of medium size, but smaller in aggregation number than the minimal population for 10^-7M. Aggregation numbers for small, medium, and large populations for 10^-9M concentration are the same as for 10^-8M, but the population is shifted to smaller aggregation number. Thus, upon dilution, an average aggregation number is gradually shifted to a smaller value. Difference in small, medium, and large particles is observed within 10^-7 - 10^-8M concentration range.

Citation Download Citation

Vadim A. Korolenko, Evgeny Evtushenko, Alexander L. Rusanov, Nadya Zubova, Ilya N. Kurochkin, and Alexander P. Savitsky "Multipopulation disaggregation behavior of zFP538 upon dilution", Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 60980N (23 February 2006); https://doi.org/10.1117/12.650601

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Proteins

Particles

Green fluorescent protein

Atomic force microscopy

Fluorescent proteins

Luminescence

Molecular aggregates

Show All Keywords

Keywords/Phrases

Search In:

Publication Years