Combination of SPR and PM-IRRAS for characterization and detection of biosensor arrays

Valdas Sablinskas; Gerald Steiner; Reiner Salzer

doi:10.1117/12.648360

23 February 2006 Combination of SPR and PM-IRRAS for characterization and detection of biosensor arrays

Valdas Sablinskas, Gerald Steiner, Reiner Salzer

Proceedings Volume 6099, Plasmonics in Biology and Medicine III; 60990I (2006) https://doi.org/10.1117/12.648360
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Functionality of biosensor arrays based on SPR imaging is mainly defined by quality of patterned SAM, which is used for sensing of biological substances of interest. The monolayers are commonly not uniform in the micrometer scale. In the current work we describe experimental setups for PM-IRRAS mapping and SPR imaging of self assembly monolayer (SAM) formed by octadecyl phosphonic acid (CH₃(CH₂)₁₇PO(OH)₂) on a patterned Au/Al₂O₃ surface. By combining highly resolved surface plasmon resonance (SPR) images of the surface with the corresponding PM-IRRAS maps we achieved enhancement of the resolution. In this work we show, that changes of molecular structure in the thin films also induce changes in the SPR signal. Contrary, changes in SPR have their origin in molecular structure within the film.

Citation Download Citation

Valdas Sablinskas, Gerald Steiner, and Reiner Salzer "Combination of SPR and PM-IRRAS for characterization and detection of biosensor arrays", Proc. SPIE 6099, Plasmonics in Biology and Medicine III, 60990I (23 February 2006); https://doi.org/10.1117/12.648360

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