Injection and manipulation of silicon microbeads in a customized microfluidic platform

Daniel Hoffmann; Des Brennan; Michael Loughran

doi:10.1117/12.660342

23 January 2006 Injection and manipulation of silicon microbeads in a customized microfluidic platform

Daniel Hoffmann, Des Brennan, Michael Loughran

Proceedings Volume 6112, Microfluidics, BioMEMS, and Medical Microsystems IV; 61120E (2006) https://doi.org/10.1117/12.660342
Event: MOEMS-MEMS 2006 Micro and Nanofabrication, 2006, San Jose, California, United States

Abstract

The first on-chip injection and manipulation of optically encoded, silicon microbeads in a microfluidic platform is reported. Encoded microbeads of different shapes and sizes were fabricated in silicon via standard microfabrication techniques. The optical signature consisted of a series of lithographically defined bar-codes, which can be identified by a laser detection system. In-situ identification of encoded microbeads was possible at microbead velocities ≤ 50 cm per second. The microbeads can also be transported within a channel network in accordance with the encoded optical signature of each bead. The microbead transport is controlled by the laminar flow of a liquid in pressure driven microchannels. Hydrodynamic pulsing facilitated single and multiple injection of microbeads from a reservoir into the laminar fluid stream of a branched microfluidic network. Careful control of the fluid velocity and alteration of the microchannel geometry also enabled manipulation of microbead velocity. The incorporation of five pillars to retain microbeads at a specific location within the microchannel network formed the basis of a reaction chamber for on chip functionalization of microbeads. The principle of hydrodynamic switching was utlized to re-direct the transport of microbeads at a branched microfluidic network. In the final part of this research we verify that this microbead technology is suitable for detection of specific target DNA.

Citation Download Citation

Daniel Hoffmann, Des Brennan, and Michael Loughran "Injection and manipulation of silicon microbeads in a customized microfluidic platform", Proc. SPIE 6112, Microfluidics, BioMEMS, and Medical Microsystems IV, 61120E (23 January 2006); https://doi.org/10.1117/12.660342

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