The interaction of lipopolysaccharide-coated polystyrene particle with membrane receptor proteins on macrophage measured by optical tweezers

Ming-Tzo Wei; Kuo-Feng Hua; Jowey Hsu; Artashes Karmenyan; Hsien-Yeh Hsu; Arthur Chiou

doi:10.1117/12.679258

14 September 2006 The interaction of lipopolysaccharide-coated polystyrene particle with membrane receptor proteins on macrophage measured by optical tweezers

Ming-Tzo Wei, Kuo-Feng Hua, Jowey Hsu, Artashes Karmenyan, Hsien-Yeh Hsu, Arthur Chiou

Author Affiliations +

Proceedings Volume 6326, Optical Trapping and Optical Micromanipulation III; 63260T (2006) https://doi.org/10.1117/12.679258
Event: SPIE Optics + Photonics, 2006, San Diego, California, United States

Abstract

Lipopolysaccharide (LPS) is one of the cell wall components of Gram-positive bacteria recognized by and interacted with receptor proteins such as CD14 on macrophage cells. Such a process plays an important role in our innate immune system. In this paper, we report the application of optical tweezers (λ = 1064nm Gaussian beam focused by a water-immersed objective lens with N.A. = 1.0) to the study of the dynamics of the binding of a LPS-coated polystyrene particle (diameter = 1.5μm) onto the plasma membrane of a macrophage cell. We demonstrated that the binding rate increased significantly when the macrophage cell was pre-treated with the extract of Reishi polysaccharides (EORP) which has been shown to enhance the cell surface expression of CD14 (receptor of LPS) on macrophage cells.

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Ming-Tzo Wei, Kuo-Feng Hua, Jowey Hsu, Artashes Karmenyan, Hsien-Yeh Hsu, and Arthur Chiou "The interaction of lipopolysaccharide-coated polystyrene particle with membrane receptor proteins on macrophage measured by optical tweezers", Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63260T (14 September 2006); https://doi.org/10.1117/12.679258

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