Time-resolved photon counting allows for new temporal and spatial insights into the nanoworld

Alexander Gaiduk; Ralf Kühnemuth; Suren Felekyan; Matthew Antonik; Wolfgang Becker; Volodymyr Kudryavtsev; Marcelle König; Fillip Oesterhelt; Carl Sandhagen; Claus A. M. Seidel

doi:10.1117/12.685366

25 October 2006 Time-resolved photon counting allows for new temporal and spatial insights into the nanoworld

Alexander Gaiduk, Ralf Kühnemuth, Suren Felekyan, Matthew Antonik, Wolfgang Becker, Volodymyr Kudryavtsev, Marcelle König, Fillip Oesterhelt, Carl Sandhagen, Claus A. M. Seidel

Author Affiliations +

Proceedings Volume 6372, Advanced Photon Counting Techniques; 637203 (2006) https://doi.org/10.1117/12.685366
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

The time-correlated single photon counting (TCSPC) technique combined with clock oscillator set by the pulsed laser provides a precise measurement of the arrival time of the detected photons with picosecond resolution for a time-scale of hours. If TCSPC is combined with other experimental techniques such as optical spectroscopy and mechanical manipulation, it is possible to coincide the detected fluorescence signal with the changes of the sample properties. High temporal resolution achieved in TCSPC (down to ps) allows us to monitor fast mechanical processes in single molecules. Here we present recent developments in fluorescence correlation spectroscopy (FCS) as well as the combination of TCSPC with optical scanning microscopy and mechanical manipulation by means of an atomic-force microscope (AFM).

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Alexander Gaiduk, Ralf Kühnemuth, Suren Felekyan, Matthew Antonik, Wolfgang Becker, Volodymyr Kudryavtsev, Marcelle König, Fillip Oesterhelt, Carl Sandhagen, and Claus A. M. Seidel "Time-resolved photon counting allows for new temporal and spatial insights into the nanoworld", Proc. SPIE 6372, Advanced Photon Counting Techniques, 637203 (25 October 2006); https://doi.org/10.1117/12.685366

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