Improvement of a fluorescence immunoassay with a compact diode-pumped solid state laser at 315 nm

Matthias Niederkrüger; Christian Salb; Michael Beck; Niko Hildebrandt; Hans-Gerd Löhmannsröben; Gerd Marowsky

doi:10.1117/12.685993

23 October 2006 Improvement of a fluorescence immunoassay with a compact diode-pumped solid state laser at 315 nm

Matthias Niederkrüger, Christian Salb, Michael Beck, Niko Hildebrandt, Hans-Gerd Löhmannsröben, Gerd Marowsky

Proceedings Volume 6380, Smart Medical and Biomedical Sensor Technology IV; 63800M (2006) https://doi.org/10.1117/12.685993
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

We demonstrate the improvement of fluorescence immunoassay (FIA) diagnostics in deploying a newly developed compact diode-pumped solid state (DPSS) laser with emission at 315 nm. The laser is based on the quasi-three-level transition in Nd:YAG at 946 nm. The pulsed operation is either realized by an active Q-switch using an electro-optical device or by introduction of a Cr⁴⁺:YAG saturable absorber as passive Q-switch element. By extra-cavity second harmonic generation in different nonlinear crystal media we obtained blue light at 473 nm. Subsequent mixing of the fundamental and the second harmonic in a β-barium-borate crystal provided pulsed emission at 315 nm with up to 20 μJ maximum pulse energy and 17 ns pulse duration. Substitution of a nitrogen laser in a FIA diagnostics system by the DPSS laser succeeded in considerable improvement of the detection limit. Despite significantly lower pulse energies (7 μJ DPSS laser versus 150 μJ nitrogen laser), in preliminary investigations the limit of detection was reduced by a factor of three for a typical FIA.

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Matthias Niederkrüger, Christian Salb, Michael Beck, Niko Hildebrandt, Hans-Gerd Löhmannsröben, and Gerd Marowsky "Improvement of a fluorescence immunoassay with a compact diode-pumped solid state laser at 315 nm", Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 63800M (23 October 2006); https://doi.org/10.1117/12.685993

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