Ultra-short pulses to signal neuronal growth cone machinery

Manoj Mathew; Ivan Amat-Roldan; Rosa Andres; Iain G. Cormack; David Artigas; Eduardo Soriano; Pablo Loza-Alvarez

doi:10.1117/12.699988

19 February 2007 Ultra-short pulses to signal neuronal growth cone machinery

Manoj Mathew, Ivan Amat-Roldan, Rosa Andres, Iain G. Cormack, David Artigas, Eduardo Soriano, Pablo Loza-Alvarez

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64410G (2007) https://doi.org/10.1117/12.699988
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Measurable change in the sensory motor machinery of growth cones are induced by non contact femtosecond laser. The focused laser beam with an average power of 3 mW was positioned at some distance away from the closest fillopodia of cortical neurons from primary cell cultures (mice E15). By identifying a set of preliminary parameters we were able to statistically analyze the phenomenological behavior of the fillopodia and classify the effects different conditions of laser light has on the growth cone. Results show that fillopodia become significantly biased towards the focused femtosecond laser light. The same experiment performed with continuous wave (CW) produced results which were indistinguishable from the case where there is no laser light present (placebo condition) indicating no clear effects of the CW laser light on the fillopodia at a distance. These findings show the potential for ultrashort pulsed light to become a new type of pathfinding cue for neuronal growth cones.

Citation Download Citation

Manoj Mathew, Ivan Amat-Roldan, Rosa Andres, Iain G. Cormack, David Artigas, Eduardo Soriano, and Pablo Loza-Alvarez "Ultra-short pulses to signal neuronal growth cone machinery", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64410G (19 February 2007); https://doi.org/10.1117/12.699988

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available