Optical tweezers force calibration using a fast shuttering camera

J. P. Sharpe; Claudia Iniguez-Palomares; Rafael Jimenez-Flores

doi:10.1117/12.696272

13 February 2007 Optical tweezers force calibration using a fast-shuttering camera

J. P. Sharpe, Claudia Iniguez-Palomares, Rafael Jimenez-Flores

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 644114 (2007) https://doi.org/10.1117/12.696272
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We report using a fast-shuttering CCD camera to determine the transverse stiffness of an optical tweezers trap. By utilizing the relation between the particle position variance and the trap stiffness we are able to circumvent the sampling requirements of the position power spectrum approach. We find that for trap stiffness of order 10^-5 N/m exposures shorter than 0.001 sec seem to be required in order to give valid measurements. For longer exposures (~0.01 sec) the trap stiffness can be overestimated by a factor of three or more.

Citation Download Citation

J. P. Sharpe, Claudia Iniguez-Palomares, and Rafael Jimenez-Flores "Optical tweezers force calibration using a fast-shuttering camera", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 644114 (13 February 2007); https://doi.org/10.1117/12.696272

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