Contrast enhancement in biomedical optical imaging using ultrabright color LEDs

Kevin Burton; Raphael Zelikowsky; Danny Shandling; Erik Lindsley; Daniel L. Farkas

doi:10.1117/12.712401

19 February 2007 Contrast enhancement in biomedical optical imaging using ultrabright color LEDs

Kevin Burton, Raphael Zelikowsky, Danny Shandling, Erik Lindsley, Daniel L. Farkas

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64411I (2007) https://doi.org/10.1117/12.712401
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The recent emergence of bright, inexpensive colored LEDs offers several advantages over traditional light sources, including reduced size and increased portability, low power consumption and heat production, increased durability and longer life, and high temporal resolution. We assembled a modular array of different Phillips LUMILED LUXEON LEDs, white and seven colors with peak wavelengths between 450 and 640 nm and bandwidths of 20-30 nm. LED illumination was fiber-optically coupled to the transmitted light path of an inverted microscope, and digital images of sectioned human tissue stained with absorbing dyes were acquired using combinations of the white and color LEDs. The LED array was also coupled to an endoscope and used to image human and mouse tissue in situ. Image contrast was assessed (1) qualitatively by looking down the microscope and by viewing the digital images, and (2) quantitatively by using entropy analysis in the real and frequency domains to assess the dependence of contrast enhancement on spatial frequency. Contrast in image features of a given color range was enhanced by LEDs conjugate to that color, whereas LED colors spanning a wider range enhanced contrast in the entire image, with white LEDs often maximizing contrast of tissue. This analysis demonstrates the utility of LED illumination in modulating contrast in light microscopy and endoscopy, which may facilitate spectral segmentation and classification of image features.

Citation Download Citation

Kevin Burton, Raphael Zelikowsky, Danny Shandling, Erik Lindsley, and Daniel L. Farkas "Contrast enhancement in biomedical optical imaging using ultrabright color LEDs", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411I (19 February 2007); https://doi.org/10.1117/12.712401

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available