Spatial difference in pH in Lucilia cuprina (Diptera: Calliphoridae) pericardial cells

Osu Lilje; Erna S. Lilje

doi:10.1117/12.699793

19 February 2007 Spatial difference in pH in Lucilia cuprina (Diptera: Calliphoridae) pericardial cells

Osu Lilje, Erna S. Lilje

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 64411Q (2007) https://doi.org/10.1117/12.699793
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Lucilia cuprina pericardial cells are primarily involved in the filtration of hemolymph. Ratio images using fluorescent pH indicator, DM-Nerf, were collected using a confocal microscope. The results support suggestions that there is zonation of cellular activity that reflect organelle distribution. Statistical analysis of the excitation ratios indicate significant spatial differences in pH of the three major zones- cortex, vacuole zone and endoplasm in pericardial cells. The outer cortex was estimated to have a pH between 5.5 and 6.8, the vacuole zone between 4.5 and 5.5 and the endoplasm between 4.0 and 5.0.

Citation Download Citation

Osu Lilje and Erna S. Lilje "Spatial difference in pH in Lucilia cuprina (Diptera: Calliphoridae) pericardial cells", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 64411Q (19 February 2007); https://doi.org/10.1117/12.699793

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