Quantitative multiplex CARS spectroscopy in congested spectral regions

Michiel Müller; Hilde A. Rinia; Mischa Bonn; Erik M. Vartiainen; Melanie Lisker; Aart van Bel

doi:10.1117/12.698566

10 February 2007 Quantitative multiplex CARS spectroscopy in congested spectral regions

Michiel Müller, Hilde A. Rinia, Mischa Bonn, Erik M. Vartiainen, Melanie Lisker, Aart van Bel

Proceedings Volume 6442, Multiphoton Microscopy in the Biomedical Sciences VII; 644206 (2007) https://doi.org/10.1117/12.698566
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

A novel procedure is developed to describe and reproduce experimental coherent anti-Stokes Raman scattering (CARS) data, with particular emphasis on highly congested spectral regions. The approach - exemplified here with high-quality multiplex CARS data - makes use the maximum entropy method for phase retrieval. The retrieved imaginary part of the nonlinear susceptibility is shown to be equal to the spontaneous Raman spectrum. The phase retrieval procedure does not influence the noise contained in the spectra. The conversion of CARS to Raman data permits a quantitative interpretation of CARS spectra. This novel approach is demonstrated for highly congested multiplex CARS spectra of sucrose, fructose and glucose. This novel procedures enables extraction of vibrational information from multiplex CARS data without the use of a priori information of the sample.

Citation Download Citation

Michiel Müller, Hilde A. Rinia, Mischa Bonn, Erik M. Vartiainen, Melanie Lisker, and Aart van Bel "Quantitative multiplex CARS spectroscopy in congested spectral regions", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 644206 (10 February 2007); https://doi.org/10.1117/12.698566

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available