Optical nano-injection into cells and 3D stem cell clusters via a NIR femtosecond laser

Iris Riemann; Frank Stracke; Aisada Uchugonova; Sven Martin; Rainer Bückle; Karsten König

doi:10.1117/12.702399

10 February 2007 Optical nano-injection into cells and 3D stem cell clusters via a NIR femtosecond laser

Iris Riemann, Frank Stracke, Aisada Uchugonova, Sven Martin, Rainer Bückle, Karsten König

Proceedings Volume 6442, Multiphoton Microscopy in the Biomedical Sciences VII; 64421F (2007) https://doi.org/10.1117/12.702399
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Multiphoton optoporation of vital cells was performed using a femtosecond pulsed laser in the near infrared (NIR). Exogenous materials such as macromolecules and exogenes were transported into the targets via laser assisted transient opening of the cell membrane. This method is also appropriate for nanoprocessing and optoporation inside 3D stem cell structures without photodestructive collateral effects which was confirmed with TUNEL-assay (DNA strand breaks) and tests for reactive oxygen species (ROS).

Citation Download Citation

Iris Riemann, Frank Stracke, Aisada Uchugonova, Sven Martin, Rainer Bückle, and Karsten König "Optical nano-injection into cells and 3D stem cell clusters via a NIR femtosecond laser", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421F (10 February 2007); https://doi.org/10.1117/12.702399

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