High-resolution surface plasmon resonance biosensing system for glucose concentration detecting

Xian Huang; Dachao Li; Haixia Yu; Fuxiang Huang; Xiaotang Hu; Kexin Xu

doi:10.1117/12.699678

15 February 2007 High-resolution surface plasmon resonance biosensing system for glucose concentration detecting

Xian Huang, Dachao Li, Haixia Yu, Fuxiang Huang, Xiaotang Hu, Kexin Xu

Proceedings Volume 6445, Optical Diagnostics and Sensing VII; 64450N (2007) https://doi.org/10.1117/12.699678
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Glucose is one of the most important substances widely contained in organism and food, thus people pay much attention in researching and improving the way for the detection of glucose. Traditional ways, although precise and reliable when in high concentration and large amount of sample, have unconvincing performance in detecting mixture and solution with low concentration and micro-volume. As far as the ideal way is concerned, it should not only specifically detect the glucose and exclude other components in solution, but also meet the need of micro-sample (approximately 5μL) and low concentration. We introduced D-galactose/D-glucose Binding Protein (GGBP) - a kind of protein which has the ability to absorb the glucose specifically, to construct a novel surface plasmon resonance measuring system. By immobilizing GGBP onto the surface of the SPR sensor, we develop a new detecting system for glucose testing in mixed solution. The experimental result indicates that compared with 0.1g/L before immobilization of GGBP, the detecting limit or the resolution of glucose testing rises to 1mg/L after the immobilization, the system succeeds in distinguishing glucose from other components in mixture, which reveals a bright future to apply SPR in the minimally invasive diabetes testing and food quality control.

Citation Download Citation

Xian Huang, Dachao Li, Haixia Yu, Fuxiang Huang, Xiaotang Hu, and Kexin Xu "High-resolution surface plasmon resonance biosensing system for glucose concentration detecting", Proc. SPIE 6445, Optical Diagnostics and Sensing VII, 64450N (15 February 2007); https://doi.org/10.1117/12.699678

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available