Fluorescence lifetime contrast in small animal imaging

V. Krishnan Ramanujan; Abhik Bandyopadhyay; Luzhe Sun; Brian A. Herman

doi:10.1117/12.701133

14 February 2007 Fluorescence lifetime contrast in small animal imaging

V. Krishnan Ramanujan, Abhik Bandyopadhyay, Luzhe Sun, Brian A. Herman

Proceedings Volume 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV; 644911 (2007) https://doi.org/10.1117/12.701133
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Early detection of primary tumors is the key for effective therapeutic intervention and successful patient survival. Small animal models emulating human diseases are powerful tools for our comprehensive understanding of the pathophysiology of tumor formation and metastasis to distant sites. Our long-term goal is to develop a non-invasive, multiphoton-fluorescence lifetime imaging (MP-FLIM) modality that can precisely quantify these steps in animal tumor models at a very early stage. The specific hypothesis is that fluorescence lifetime can be employed as reliable contrast parameter for providing higher detection sensitivity as compared with conventional intensity-based tumor imaging approaches and therefore it is possible to detect smaller tumor volumes (early detection) than those achieved by other prevailing methods. We base this hypothesis on our recent observations that (1) fluorescence lifetime is "intrinsic" to the fluorophore and its measurement is not affected by concentration and/or spectral artifacts as in intensity-based methods, (2) multiphoton excitation can enable increased tissue penetrability and reduced phototoxicity and (3) MP-FLIM approach can discriminate background autofluorescence from the fluorescent proteins in thick tissues thereby achieving a ten-fold increase in signal-to-background ratio over the intensity-based approaches. We present our preliminary data to support this hypothesis in primary tumor detection in nu/nu athymic mouse models.

Citation Download Citation

V. Krishnan Ramanujan, Abhik Bandyopadhyay, Luzhe Sun, and Brian A. Herman "Fluorescence lifetime contrast in small animal imaging", Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 644911 (14 February 2007); https://doi.org/10.1117/12.701133

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KEYWORDS

Tumors

Luminescence

Tissues

Fluorescence lifetime imaging

Imaging systems

Mouse models

Preclinical imaging

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