Role of pH in the appearance of the fluorescent state of chromo protein asCP595 and its mutant KFP

Alexander L. Rusanov; Nadya Zubova; Alexander P. Savitsky

doi:10.1117/12.717411

14 February 2007 Role of pH in the appearance of the fluorescent state of chromo protein asCP595 and its mutant KFP

Alexander L. Rusanov, Nadya Zubova, Alexander P. Savitsky

Proceedings Volume 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV; 644917 (2007) https://doi.org/10.1117/12.717411
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

At the present time the phenomenon of "fluorescence kindling," which is typical of asCP595 protein and some of its mutants, is of great interest for the studies of intracellular traffic. The range of changing of intracellular pH may be rather wide, however, the effect of pH on the fluorescent state of this protein is poorly investigated. Our studies have revealed that the fluorescence intensity of asCP595 increases significantly in alkaline conditions. In addition, the observed change in the position of excitation, emission, and absorption maxima indicates the appearance of new spectral forms of the protein. These conformers are characteristics of new fluorescent states. The changes in the absorption spectrum are indicative of a new dark form of the protein at alkaline pH.

Citation Download Citation

Alexander L. Rusanov, Nadya Zubova, and Alexander P. Savitsky "Role of pH in the appearance of the fluorescent state of chromo protein asCP595 and its mutant KFP", Proc. SPIE 6449, Genetically Engineered and Optical Probes for Biomedical Applications IV, 644917 (14 February 2007); https://doi.org/10.1117/12.717411

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