Toward the standing wave surface plasmon resonance fluorescence microscopy

Euiheon Chung; Wai Teng Tang; Yang-Hyo Kim; Colin J. R. Sheppard; Peter T. C. So

doi:10.1117/12.705720

14 February 2007 Toward the standing wave surface plasmon resonance fluorescence microscopy

Euiheon Chung, Wai Teng Tang, Yang-Hyo Kim, Colin J. R. Sheppard, Peter T. C. So

Proceedings Volume 6450, Plasmonics in Biology and Medicine IV; 64500A (2007) https://doi.org/10.1117/12.705720
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Surface plasmons are coherent oscillations of the free electrons on metal surface which can be used to improve the excitation efficiency of fluorophores due to increased field enhancement. Surface plasmon resonance fluorescence (SPRF) microscopy is a wide-field optical imaging technique that utilizes the evanescent electromagnetic field of surface plasmons to excite fluorophores near to a surface of a metal film. With the same excitation power, the field enhancement effect of the surface plasmon resonance (SPR) leads to strong fluorescence emission and thus increases the signal to noise ratio of detection. However, there have been few studies on the image formation process for SPRF in terms of its point-spread function. By imaging fluorescent microspheres with size below the diffraction limit, we obtained the point-spread function for SPRF. The SPR enhancement is confirmed by back-focal-plane imaging with various incidence angles of the excitation beam. Furthermore, we will investigate the potential of resolution enhancement by generating standing wave with two symmetric incident excitation beams toward the standing-wave surface plasmon resonance fluorescence (SW-SPRF) microscopy.

Citation Download Citation

Euiheon Chung, Wai Teng Tang, Yang-Hyo Kim, Colin J. R. Sheppard, and Peter T. C. So "Toward the standing wave surface plasmon resonance fluorescence microscopy", Proc. SPIE 6450, Plasmonics in Biology and Medicine IV, 64500A (14 February 2007); https://doi.org/10.1117/12.705720

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KEYWORDS

Surface plasmons

Point spread functions

Microscopy

Luminescence

Metals

Resonance enhancement

Glasses

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