Integrated ultraviolet and tunable mid-infrared laser source for analyses of proteins

Hisanao Hazama; Yoshiaki Takatani; Kunio Awazu

doi:10.1117/12.700112

14 February 2007 Integrated ultraviolet and tunable mid-infrared laser source for analyses of proteins

Hisanao Hazama, Yoshiaki Takatani, Kunio Awazu

Proceedings Volume 6455, Nonlinear Frequency Generation and Conversion: Materials, Devices, and Applications VI; 645507 (2007) https://doi.org/10.1117/12.700112
Event: Lasers and Applications in Science and Engineering, 2007, San Jose, California, United States

Abstract

Mass spectrometry using matrix-assisted laser desorption/ionization (MALDI) technique is one of the most widely used method to analyze proteins in biological research fields. However, it is difficult to analyze insoluble proteins which have important roles in researches on disease mechanisms or in developments of drugs by using ultraviolet (UV) lasers which have commonly been used for MALDI. Recently, a significant improvement in MALDI process of insoluble proteins using a combination of a UV nitrogen laser and a tunable mid-infrared (MIR) free electron laser (FEL) was reported. Since the FEL is a very large and expensive equipment, we have developed a tabletop laser source which can generate both UV and tunable MIR lasers. A tunable MIR laser (5.5-10 &mgr;m) was obtained by difference frequency generation (DFG) between a Nd:YAG and a tunable Cr:forsterite lasers using two AgGaS₂ crystals. The MIR laser can generate pulses with an energy of up to 1.4 mJ at a repetition rate of 10 Hz. A UV laser was obtained by third harmonic generation of a Nd:YAG laser splitted from that used for DFG. A time interval between the UV and the MIR laser pulses can be adjusted with a variable optical delay.

Citation Download Citation

Hisanao Hazama, Yoshiaki Takatani, and Kunio Awazu "Integrated ultraviolet and tunable mid-infrared laser source for analyses of proteins", Proc. SPIE 6455, Nonlinear Frequency Generation and Conversion: Materials, Devices, and Applications VI, 645507 (14 February 2007); https://doi.org/10.1117/12.700112

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