Attenuation of molecular function by multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) using green fluorescent protein

Tetsuro Takamatsu

doi:10.1117/12.709235

13 March 2007 Attenuation of molecular function by multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) using green fluorescent protein

Tetsuro Takamatsu

Author Affiliations +

Proceedings Volume 6460, Commercial and Biomedical Applications of Ultrafast Lasers VII; 646004 (2007) https://doi.org/10.1117/12.709235
Event: Lasers and Applications in Science and Engineering, 2007, San Jose, California, United States

Abstract

Noninvasive and straightforward methods to inactivate selected proteins in the living cell with high spatiotemporal resolution are eagerly sought for elucidation of protein function in the post-genome-mapping era. Chromophore-assisted laser inactivation (CALI) facilitates inactivation of proteins by photochemically generated reactive oxygen species (ROS), but CALI using single-photon excitation thus far has presented several drawbacks, including its complex procedure, low efficiencies of inactivation with a certain chromophore, and photodamage effects. We here show that by application of multiphoton excitation to CALI using near-infrared femtosecond laser, enhanced green fluorescent protein (EGFP) can work as an effective chromophore for inactivation of a protein's function without nonspecific photodamage in the living cell.

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Tetsuro Takamatsu "Attenuation of molecular function by multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) using green fluorescent protein", Proc. SPIE 6460, Commercial and Biomedical Applications of Ultrafast Lasers VII, 646004 (13 March 2007); https://doi.org/10.1117/12.709235

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