Time-resolved evanescent wave-induced fluorescence studies of macromolecular adsorption

Trevor A. Smith; Colin A. Scholes; Michelle L. Gee

doi:10.1117/12.699637

9 February 2007 Time-resolved evanescent wave-induced fluorescence studies of macromolecular adsorption

Trevor A. Smith, Colin A. Scholes, Michelle L. Gee

Proceedings Volume 6483, Complex Light and Optical Forces; 64830C (2007) https://doi.org/10.1117/12.699637
Event: Integrated Optoelectronic Devices 2007, 2007, San Jose, California, United States

Abstract

Evanescent Wave (EW) fluorescence spectroscopic techniques are exploited to obtain information about the conformational state of macromolecules within close proximity of a solid/liquid interfacial region. Specifically, timeresolved evanescent wave-induced fluorescence techniques have been applied to the study of the adsorption of polymers and biomolecules to silica surfaces. We have extended these EW measurements using polarized excitation and emission detection to probe molecular motion and conformational change in the microenvironment of the interfacial region. We report on the observation of complex time-dependent fluorescence anisotropy data and the interpretation of these data in terms of in- and out-of-plane rotational motion. The macromolecular-interfacial systems investigated by this evanescent wave approach included polymer film dynamics and adsorbed protein rearrangement upon adsorption.

Citation Download Citation

Trevor A. Smith, Colin A. Scholes, and Michelle L. Gee "Time-resolved evanescent wave-induced fluorescence studies of macromolecular adsorption", Proc. SPIE 6483, Complex Light and Optical Forces, 64830C (9 February 2007); https://doi.org/10.1117/12.699637

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