Measuring of optical properties of biological samples by low cost goniometrical equipment

M. Hoffmann; O. Schewtschenko; O. Minet

doi:10.1117/12.740805

26 April 2007 Measuring of optical properties of biological samples by low cost goniometrical equipment

M. Hoffmann, O. Schewtschenko, O. Minet

Proceedings Volume 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII; 65350I (2007) https://doi.org/10.1117/12.740805
Event: Saratov Fall Meeting 2006, 2006, Saratov, Russian Federation

Abstract

The determination of the optical parameters is of importance to the analysis of turbid media. In combination with mathematical modelling, these data are a starting point for optical diagnosis methods in medicine, for quality assurance in the food industry as well as in environmental monitoring. Turbidimetry, 90°-light scattering measurement and light scattering goniophotometry have, meanwhile, become well-established methods. Usually, goniophotometry methods are used for determination of the scattering phase function in media with low concentrations of the suspended particles. By means of our experimental arrangement we show that the optical parameters of media with high concentrations of the suspended particles can be measured using the goniophotometer, too. The mathematical analysis of our data is based on a data base which was generated by means of extensive Monte Carlo simulations. An adapted least square method allows an on-line analysis. For demonstration of the effectiveness of the arrangement, homogenized whole milk and milk diluted with water in several concentrations was analysed. Optical parameters of milk were calculated and compared with the literature data.

Citation Download Citation

M. Hoffmann, O. Schewtschenko, and O. Minet "Measuring of optical properties of biological samples by low cost goniometrical equipment", Proc. SPIE 6535, Saratov Fall Meeting 2006: Optical Technologies in Biophysics and Medicine VIII, 65350I (26 April 2007); https://doi.org/10.1117/12.740805

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KEYWORDS

Scattering

Light scattering

Monte Carlo methods

Goniophotometry

Absorption

Geometrical optics

Particles

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